Journal: iScience

Article Title: Scanning Bessel beam microscopy with a protected and corrective objective for solvent-cleared large samples

doi: 10.1016/j.isci.2026.116358

Figure Lengend Snippet: High-resolution autofluorescence imaging of CUBIC-R-cleared E15.5 mouse embryos and Thy1-YFP-H mouse brains (A) Maximum intensity projection of a whole CUBIC-R-cleared E15.5 mouse embryo. (B) Surface rendering of a Thy1-YFP-H transgenic mouse brain. (C) Whole-brain image with depth-coded fluorescence intensity. A magnified inset shows the labeled neuron. (D) Surface rendering of the E15.5 mouse embryo. (E–G) Digital sections extracted from the 3D image along the sagittal (E), coronal (F), and transverse (G) planes. Scale bars: 1 mm.

Article Snippet: CUBIC-R+ (for animals) , Tokyo Chemical Industry , Cat#T3740.

Techniques: Imaging, Transgenic Assay, Fluorescence, Labeling

Journal: iScience

Article Title: Scanning Bessel beam microscopy with a protected and corrective objective for solvent-cleared large samples

doi: 10.1016/j.isci.2026.116358

Figure Lengend Snippet: High-resolution light sheet autofluorescence imaging of a CUBIC-R-cleared E15.5 mouse embryo (A–D) Representative sagittal digital sections extracted from a reconstructed 3D image of the whole embryo. (E–I) High-resolution views of anatomical structures, including (E) vibrissal follicles, (F) heart, (G) lung, (H) tongue, and (I) kidney. (J–L) Visualization of early nephron development in the embryonic kidney. (M) Whole kidney autofluorescence image with color-coded depth information. (N) Image segmentation showing three individual nephrons. (O) Capillary walls (purple) inside Bowman’s capsule. (P) 3D rendering of segmented nephrons illustrating their spatial morphology.

Article Snippet: CUBIC-R+ (for animals) , Tokyo Chemical Industry , Cat#T3740.

Techniques: Imaging

Journal: iScience

Article Title: Scanning Bessel beam microscopy with a protected and corrective objective for solvent-cleared large samples

doi: 10.1016/j.isci.2026.116358

Figure Lengend Snippet: Autofluorescence imaging of squid embryos at developmental stages 24 and 27 Squid embryos at stage 24 and 27 were prepared using CUBIC-R tissue clearing and imaged with capped 4× objective. (A) Surface rendering of a stage 24 embryo generated via AMIRA, illustrating the structural details of the sample surface. (B and C) Dorsal and lateral views of stage 27 embryos, respectively. At this stage, the mantle and body are well-developed, with the yolk clearly connected to the head and mouth regions. (D and E) Autofluorescence section of the embryonic body, highlights the key anatomical features of above figures. The early eye and neural development at stage 24, optic lobe and arm primordia formation at stage 27. The inset is the segmentation reconstructed manually shown in 2×. The arm is manually segmented with a blue lines mask and shown in (G) and (H). (F) The autofluorescence image of the lateral view of the stage 27 squid embryos. The magnified inset highlights the structural linkage between the embryonic body and the yolk sac. Manual segmentation reveals that this connection consists of three major bundles originating from the yolk. The diameter each bundle is about 15 ± 5 μm, and total width of the three-bundle is about 110 μm. (G) Surface rendering of the stage 27 squid embryo arms, and (H) representative optical sections showing manual segmentations. The sucker bulb can be observed and segmented to reconstruct the structure. The arm diameter is from 204 ± 3 (upper left) to 306 ± 5 (lower right) μm, measured by the ImageJ.

Article Snippet: CUBIC-R+ (for animals) , Tokyo Chemical Industry , Cat#T3740.

Techniques: Imaging, Generated